FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597649
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.041.5102048
GTCTCGC11600.037.8068581
CTGTCGC15100.036.6588759
TCGCTCT13850.032.180954
CTATACG1204.2664792E-429.9533714
GTCTTGC26050.028.9787961
GTATAGG7300.028.561031
TCTCGCT15800.027.7542652
GTGTAAC7550.027.6153031
GCGCTAC2105.3708754E-727.3885751
CGCTCTG15150.027.0470035
GTATACG1907.642771E-626.4876371
GTTTCAC42650.026.1258013
TAATACT8400.025.6743164
GGGTTTC46250.025.649031
GTATTAG9300.025.5111331
CTCGCTC16400.025.4238363
CCGTGTT9000.024.761939
TGTACCG2101.6618682E-523.9626965
GTCTCAC32200.023.8905811
Produced by FastQC (version 0.11.5)