FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400151
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.035.315978
CTGTCGC14800.032.0598269
GTACGGC1402.9106586E-530.8113841
GTCTTGC22750.030.653381
TTCACCG6900.028.1315485
GTCTCGC11000.028.1037181
ACCGTGT8500.026.2198858
TCGCTCT13100.025.7932194
ACTACCG1450.00128869924.7902535
CCGTGTT8350.024.1074079
TAGTGCG1500.001569854123.964417
TTGCTCT33950.023.928624
GTATAGG5600.023.1085381
TCTCGCT13700.023.0888352
GTATAAT7850.022.8959331
CTCGCTC14150.022.862623
GTACAAC6950.022.7575681
GTACTAG6050.022.578041
CGCTCTG14650.022.0827855
TCACCGT9450.022.0624716
Produced by FastQC (version 0.11.5)