FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559724
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10950.036.820658
GTCTCGC12000.036.6252061
GTCTTGC25450.033.1229931
CTGTCGC13100.032.9759649
TCGCTCT13150.030.660544
CTCGCTC13550.030.3027613
TCTCGCT13800.029.2318022
GGTTACG1753.965444E-628.8198381
CGCTCTG14750.027.334655
TTACGCT2051.3627863E-524.5845184
GGTATAC6800.024.3697151
GTGTTAG17750.023.5429631
GTTTCAC41650.023.0029353
CGTACTC1600.00225888222.5110973
GGGTTTC46100.022.036861
GTCTCAC30800.021.9891621
GGGTACG2954.1192106E-721.9812321
GTGTTAT10850.021.9137021
GTATTGA10850.021.9137021
TTGCTCT37050.021.7644334
Produced by FastQC (version 0.11.5)