FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559724
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11750.037.9346581
GTCTTGC24650.037.6231461
TCTGTCG9000.037.543028
TCGCTCT12650.030.1202934
GTCTTAC9450.029.6698341
GTATTAA8250.027.8854921
GTATTAC7050.027.5332161
CTCGCTC13750.027.1878263
GTACTAG5600.026.9596081
CTGTCGC13350.026.9254259
TCTCGCT15850.026.7606472
GGTACGC1400.001050217725.6758171
CGCTCTG14250.025.224875
GTGTTAA12050.025.0578941
GGGTTTC43850.024.4286231
TTGCTCT36200.024.228424
TTACACT13850.023.358054
GTGTTAT11700.023.3496321
TTAACGC1550.001900262423.1906073
GTGTTAC10900.023.084681
Produced by FastQC (version 0.11.5)