FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493240
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10400.038.7117841
TCTGTCG10500.036.288288
GTCTTGC23850.032.555461
TATACCG900.003782079831.9519425
TCGCTCT12800.031.4526964
CTGTCGC13050.028.646579
TCTCGCT13850.028.0300432
CTCGCTC13900.027.4120083
GCGTTAC1050.00803334427.3879281
GTATTAC5600.026.9599911
GTCTCAC29150.025.4031281
CTCGATC9500.024.9729658
GTCTTAC11300.024.8127351
TTACACT12800.024.7128314
GTATTAG10150.024.0824891
GTGTTAG15450.023.2664451
CGCTCTG15500.023.1909265
GTATACG1550.001900097623.1909263
TTGCTCT32200.022.9964684
GTATTAA8150.022.935291
Produced by FastQC (version 0.11.5)