FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557993
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11750.042.2186788
GTCTCGC11350.039.9044341
CTGTCGC15200.036.893049
TCGCTCT12500.035.0829624
TCGACCG850.002857968733.8325279
GTCTTGC26800.031.6536391
GTATTAC6300.030.8105661
TCTCGCT13800.030.215192
CTCGCTC14350.028.5561313
CGCTCTG14900.027.5020485
GTTTCAC37100.026.5474663
GTATTAA8200.026.3017021
GGGTTTC41600.026.0951651
GTGTAAT10450.024.7663881
TACCCGT2051.3784611E-524.5482565
GTCTTAC10450.024.0784321
TTCACCG7250.023.7985045
GTACTAC5200.023.5029321
GTGTTAC11650.022.8324341
GGTATAC5050.022.7774471
Produced by FastQC (version 0.11.5)