FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610741
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12650.044.4303361
TCTGTCG10600.042.111778
TCGCTCT12600.041.7128684
TCTCGCT13950.039.764192
CTCGCTC14400.036.520443
GTCTTGC25950.036.0978621
CTGTCGC13650.035.3394559
CGCTCTG15550.028.7064195
TAATACT8100.027.5546194
TTGCTCT33000.027.27184
GGGTTTC47100.026.7727281
GTTTCAC44750.026.7233353
GTGTAAA17150.026.469881
GTACTAC4850.025.2570531
TCTTGCT37750.024.0452442
CTTGCTC36100.023.5478063
GGTTTCA47000.023.4514222
TTACACT11900.022.9908124
TTCACCG8200.022.8284915
GTATTAC5700.022.7548071
Produced by FastQC (version 0.11.5)