FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610741
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12600.052.4902958
GTCTCGC12550.046.972121
TCGCTCT13400.039.699834
CTGTCGC16700.039.172989
GTCTTGC27750.036.7870861
TCTCGCT16100.033.9351232
CGCTCTG14850.033.4029125
CTCGCTC15400.032.6767653
TATACCG1000.00633275128.755555
GTAACGC1306.8148086E-427.649573
TTGCTCT35250.026.3082714
GTATATA12000.025.7606751
TACCGTA1400.001050513925.6745997
GTCTTAC10700.025.5311181
TTACACT15150.024.6747324
TACACTG19350.024.5202375
CTTGCTC35450.024.3347423
CTGTTGC29600.024.2867839
GTATTGC7000.023.6210841
GGGTTTC43700.023.3602221
Produced by FastQC (version 0.11.5)