FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2825516
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11000.041.889858
GTCTCGC13500.039.497121
GTCTTGC25900.035.0540541
TCGCTCT14150.034.599834
CTGTCGC16550.033.4976439
TCTCGCT15800.032.3707962
GTATTAT9600.030.7737271
CGCTCTG15550.029.632695
CTCGCTC15900.028.542733
GTCTTAC10450.027.5810741
TAATACT7850.027.5152244
GTACTAA5800.027.3314191
GTATTAC6650.027.0885541
GTGTAAC9050.024.6820141
GGGTTTC51700.024.529551
GTCTCAC32600.024.3132251
GTATTAG10850.023.9078351
GTACTGA9950.023.8978231
TTGCTCT39400.023.7557434
GTGTTAC11250.023.6982731
Produced by FastQC (version 0.11.5)