FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895702
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.044.826858
GTCTCGC12450.039.541551
TCGCTCT12900.036.2751164
CTGTCGC14850.033.4520579
GTCTTGC24800.030.067661
TCTCGCT15300.029.644852
CGCTCTG14550.029.1926965
CTCGCTC15700.028.8895683
GTACCGC2156.2087383E-726.9380171
GTGTTAC11200.025.2092951
GTGTAAA13650.024.3971611
GTCCTAC8500.023.848071
GTCTCAC29200.023.80141
GTCTTAC9450.023.7489261
TTGCTCT30950.023.4933994
GGGTAAT9450.022.9828321
GTGTTAG19600.022.9007511
GTGTAAG13350.022.7762451
GTGTAGT16000.022.6237241
GTATTAA7400.022.5014361
Produced by FastQC (version 0.11.5)