FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2535818
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9850.042.607771
TCTGTCG9600.041.247668
TCGCTCT10500.037.7114074
TCTCGCT11700.035.6909872
GTCTTGC20100.032.3998871
CTCGCTC12300.032.193933
CCGTACT1402.8828723E-530.8547844
CGCTCTG12700.030.044955
CTGTCGC14300.029.7046099
TCCGTAC1952.7739588E-729.537373
GTCTCAC24700.028.7095361
GTGTAAA13500.027.8719021
TTACACT10650.027.716194
GTATAAT6950.027.0698321
TCTCGAT9200.026.6071857
TCGATCT9250.026.4633669
GTGTTAC8500.026.3900261
TACACTG13700.026.2753665
GTATTAA7100.025.4787851
GTGTTAG15450.025.2907891
Produced by FastQC (version 0.11.5)