FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2535818
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10350.047.2952088
GTCTCGC10550.040.6406751
TCGCTCT12450.038.744854
GTCTTGC20550.036.7775571
CTGTCGC15150.036.5884329
CGCTCTG13350.031.2754735
CTCGCTC13700.031.0074213
TAATACT7400.030.1604964
TCTCGCT13450.029.9985242
GTATTAC5050.027.341581
TTGCTCT27150.026.783054
TCTGTCA26000.026.3026088
GTGTAAG11250.025.8385931
GTTTCAC29000.025.8207913
GTGTAAT9300.025.005091
GGGTTTC31150.024.7291531
GTCTCAC24750.024.3704911
GGAGTGC50050.023.7321475
GTGTTAT7750.023.4422721
CCGTACT1851.920417E-423.3500614
Produced by FastQC (version 0.11.5)