FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963613
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11850.040.6988038
GTCTTGC21750.037.465571
GTCTCGC11950.035.9217641
GTGTTAC10650.031.4254681
GTCTTAC9600.030.315331
CTCGCTC14600.030.0818653
TCGCTCT14850.029.5739384
CTGTCGC17250.028.3760769
CGCTCTG15550.028.2383485
TTGCTCT31200.026.7675844
TCTCGCT15300.026.3704532
TTACACT11900.024.8051974
ACCGTGT8250.024.4303258
GGCTAGA15600.024.2522641
GTCTCGA23900.024.0952786
GTATAGG8150.023.2107541
TACACTG15250.023.129495
GTTTCAC34850.022.7257213
CTCGATC12700.022.671568
TTCACCG7650.022.5834145
Produced by FastQC (version 0.11.5)