FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477214
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9050.037.3886268
GTCTCGC9600.034.699761
TCGCTCT9800.034.527254
GTCTTGC17750.034.2705271
TACCCGT1601.9843483E-631.4969315
GTATTAA8450.029.1381531
CTGTCGC12000.028.7971959
GTATTAC6750.027.893911
TCTCGCT11450.027.0361212
CTCGCTC11750.026.3458393
GTGTAAA13850.024.5746841
CGCTCTG12350.024.4834465
GTGTTAT10100.024.3779621
TTGCTCT27950.024.212314
GTATAGA8700.024.1389621
ATACCCG1801.5953304E-423.9976634
GTATTGG8200.023.8445851
GTTAGCC12150.023.7009163
CTTGCTC25100.023.5191483
GTGTTAG14500.023.4730591
Produced by FastQC (version 0.11.5)