FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477214
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10150.039.423051
TCTGTCG10550.036.8425988
CTCGCTC11850.034.6307533
TCGCTCT12550.034.418084
CGCTCTG12950.033.3495865
TCTCGCT13500.032.014812
GTCTTGC19000.030.633031
GTATTAC4700.029.4109691
CTGTCGC12900.028.4587449
GTATAAT8700.027.5961361
GTGTTAG14000.027.5423771
GTGTTAC9800.026.7257561
GTGTAAA15250.026.716021
TTGCTCT24600.025.752934
GCGTTAT2001.0378179E-525.4637071
GTACAAT8300.025.4198781
GTCTCAC24900.024.8355161
GTATTGG7150.024.4207381
TTTACCG1500.001557478223.9970474
TACTTCG1500.001559309823.9922057
Produced by FastQC (version 0.11.5)