FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244487
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGGAGTAATCTCGTATGCCGT50190.22361457206034166No Hit
TCTCCGAGCCCACGAGACAAGGAGTAATCTCGTATGCCGTCTTCTGCTTG28540.1271560049133722Illumina PCR Primer Index 5 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA7750.026.154261
GTACTAA2507.9546226E-826.0608521
TTATACT3057.730705E-1025.9652374
GTCTTAC4500.025.7391131
GTATTAG4400.024.6788371
GTCTTGC16600.024.4211461
TCTGTCA14550.023.7512978
GGTTAAT4303.6379788E-1223.5692441
GTATAAG8300.023.5489641
TTAAACG1851.9205935E-423.3495754
TCACTCT16650.022.4847744
TAACCTA2003.2550457E-421.5983565
GTGCATA4701.0913936E-1121.5633531
GTCTCAC13150.021.4696481
GTTTTAC5450.021.252481
GTGTAAC3757.912604E-921.2347681
GTATTAA3809.216819E-920.9553621
GTCTCGC13650.020.6832161
GTACTAC2455.177126E-520.6832141
TCTGTCG8400.020.5703228
Produced by FastQC (version 0.11.5)