FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244487
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAG7600.027.766681
GTTAGCC7650.027.295033
GTATTAA3601.7644197E-1024.2559531
GTACTAC2701.5929618E-724.2559531
GTCTCGC13800.024.255951
TCTGTCG10750.023.4337658
TGTTAGC8700.023.190762
GGTATAC2852.6847556E-722.9793221
GTATAAG5700.022.9793221
GTACTAA2903.1749187E-722.5831281
GGTCTAT6200.022.2998281
GTTTAAA7200.022.2346231
GTGCTAA4659.094947E-1221.9086021
GTTCTAC4451.09139364E-1021.2580241
GTCTTAC5251.8189894E-1220.7908151
GGGTAAC5353.6379788E-1220.4022031
TCGCTCT12550.020.0781154
GTCTAGC5103.092282E-1119.9754891
ATACGGT2908.763251E-619.8627323
GTCTAAC3301.099681E-619.845781
Produced by FastQC (version 0.11.5)