FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268589
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10700.041.0431378
GTCTTGC24800.037.0918771
TCGCTCT14000.034.9672974
GTCTCGC13900.032.307521
CTCGCTC15650.030.821113
CTGTCGC15000.030.2373549
TCTCGCT14750.029.2851262
CGCTCTG15800.027.3385435
TACCCCG2405.6523277E-826.996815
GTCTCAC31300.026.8435781
GTCTTAC11500.026.45321
TAATACT9900.026.1787264
GTGTTAG21800.025.9158151
GTATTAT9900.025.6070541
GTATTAC7750.025.2341581
TTGCTCT32250.025.2249284
GTTCTAA10650.025.1639541
GTACTAT6800.024.498851
GGAGTGC66900.023.6743325
GTGTAAT14900.023.3335971
Produced by FastQC (version 0.11.5)