FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018932
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12650.035.8495568
GTCTCGC10650.034.182961
GTCTTGC23000.032.6060871
TCGCTCT12950.029.464394
CGCTCTG14000.028.2797575
CTCGCTC13900.027.9704273
GTCTTAC10150.026.5414681
CTGTCGC16700.026.2934239
GGTATAC5650.025.7733461
TACACTG15850.025.4331265
GTGTTAG19350.025.2105941
TCTCGCT15700.024.3215582
GTGTTAC11200.023.4031181
GTGTACG3752.928573E-1023.2991051
GTATTAA8450.023.2646371
ACCGTGT8750.023.0347618
GTCTAGA13350.022.9064241
TTGCTCT31250.022.577064
GTCCTAA10000.022.5710091
GTATTAT7800.021.4695261
Produced by FastQC (version 0.11.5)