FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702860
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA1002.8709455E-643.1963466
GTCTCGC9950.040.7392581
TCTGTCG9650.038.0485958
TCGCTCT11900.036.9037634
TCTCGCT11900.032.6689032
GTCTTGC24050.031.301591
CTGTCGC13300.029.2306149
TCGGTAC1000.00628892628.7964973
CGCTCTG13300.028.6887745
CTCGCTC13500.028.2632313
TTGCTCT28750.026.2929444
GTCTTAC9400.026.1817841
GTGTTAG19450.025.3068141
CTTGCTC28500.025.2600863
GTCTCAC25850.025.2017171
GTATTAG9500.025.144241
GTATTAA8650.025.1045991
GTGTATA15100.024.447891
TTCACCG7400.024.3218155
GTGTTAC10100.023.6505221
Produced by FastQC (version 0.11.5)