FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702860
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10550.039.3044931
TCTGTCG10900.035.6574948
GTCTTGC22250.035.311291
GTACCGG1402.6850572E-531.1776261
CTCGCTC11850.030.985893
GTCTTAC10150.030.8192621
GTGTTAG14750.030.085531
TCTCGCT12950.030.0401022
GTATTAG7800.029.845251
TCGCTCT12350.029.7291984
GTGTAAC6850.028.674311
CGCTCTG13300.027.0593915
CTGTCGC16150.026.7410459
TTGCTCT29450.026.4008854
GTGTAAG11700.026.1145921
TTACACT12350.025.648724
GTATAAC5350.024.4758931
CTTGCTC28300.024.1684763
GTGTAGG12350.024.1510891
GTATTAA7850.023.1680891
Produced by FastQC (version 0.11.5)