FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159536
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9850.038.007528
GTCTTGC26500.034.6888241
TCGCTCT12600.031.4263974
CTGTCGC13500.028.264719
GTGTTAC10900.027.8903871
GTCTCGC12350.027.5462571
GTCTTAC10800.027.4784241
GTGTAAA17800.026.8383391
TAACGCT3006.384653E-1026.398174
CTTGCTC32100.025.3456423
TTGCTCT32500.025.0321124
TTACTCT15500.024.1531664
GCGTTAC2101.5761701E-524.1273961
GGGTTTC44600.023.8569091
TATACTG10300.023.7653455
GTCTAGC12900.023.5662941
CGCTCTG13650.023.2071845
TTACACT14050.023.0589034
GTGCTAC10150.022.8200021
TACACTG16950.022.5117155
Produced by FastQC (version 0.11.5)