FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159536
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11800.036.599378
GTCTCGC12600.031.7555071
GTCTTGC25100.031.0125141
GTATTAT10350.030.9271051
GTCTTAC10700.027.8757761
GTGTAAG14300.026.962911
GTCTTAG11050.024.3593851
GTGTTAC11400.024.2496591
TCGCTCT15500.024.1511524
CTGTCGC18500.024.1237539
TTGCTCT32800.023.9231784
GTATAAG8700.023.4134651
CGCTCTG15700.023.3827445
GTATTAG9700.023.2496761
GTACTGG14100.023.217761
CTCGCTC15900.022.6387673
GTATTGT10650.022.5419371
CGTTTCG2252.8049682E-522.3944539
CTTGCTC32800.022.3875353
GTATTAC7800.022.38431
Produced by FastQC (version 0.11.5)