FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218350
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7950.041.6589138
CGTTTCG800.002107894735.998739
TCGCTCT9800.033.060064
GTCTTGC18100.031.4243091
GTCTTAC6800.030.7048021
GTGTAAC7550.030.5154821
GTCTCGC10100.029.939541
GTATTAC4850.029.6896761
CTGTCGC13850.027.0315389
GTATTAG7850.026.597791
CGCTCTG10900.026.4210875
TCTCGCT11950.025.9070362
ACCCGTT2259.685973E-725.5990986
TAATACT5350.025.5691934
GTGTTAC7350.025.468491
TACCCGT1701.0817862E-425.4108725
CTCGCTC11500.024.4165333
GTCTCAC23750.024.2517781
GTATAAG7800.023.9991531
TATACTG6950.023.82655
Produced by FastQC (version 0.11.5)