FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218350
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9100.038.0097581
TCTGTCG8300.034.697268
GTCTTGC17400.030.6462761
GTGTTAG12950.030.6047021
TCGCTCT9700.030.4316464
TACCCCG1703.1819618E-629.645755
GTATATA8850.029.312611
GTACTAG3700.029.213581
GTATTAA6400.028.14851
TAGCGCT2105.304282E-727.4273624
GTGTAAG10350.027.1531091
CTCGCTC11000.026.835183
GTCTTAC8250.026.2036971
GGTATAC4400.026.2036951
TCTCGCT11300.026.123332
GTGTAAA11350.026.0305461
CGCTCTG11200.025.713155
GTAACGC1400.001041309825.713153
GTATACG2001.117753E-525.2210561
GTGTTAC8300.025.1776491
Produced by FastQC (version 0.11.5)