FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515116
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8700.038.067948
GTCTCGC9850.035.8505021
GTCTTGC22050.032.3565371
TCTCGCT13000.027.1382712
GTATAAA11450.027.06441
TTGCTCT26900.026.7650784
TCGCTCT12050.026.2897474
GTATTAC5500.026.2061211
TATACTG7500.025.9193045
CTGTCGC14700.025.46879
CTCGCTC13200.025.0902353
GTGCTAC7900.024.6304341
GTGTAAG12100.024.4193381
GTGTAAT8950.024.1564791
GTACTAG3902.1827873E-1124.0222781
TACCGGA1500.001556433523.9998322
GTATTAG7850.023.8692671
TAACGCA2151.9702991E-523.4412294
CTTGCTC25250.023.3815483
CTATTAC5000.023.0613861
Produced by FastQC (version 0.11.5)