FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927953
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10850.036.4967421
GTCTTGC25000.034.2711031
TCTGTCG11100.033.7288488
TCGCTCT11150.032.9318774
TCTCGCT13150.027.3757112
GTGTTAC10900.027.0818611
GTCCTAC7900.025.5183221
CTCGCTC13650.025.3180223
TTGCTCT32000.025.1993434
GTGTTAG18700.024.2560521
GTATTAA8700.023.9993741
GACCCGT2701.7657476E-723.9993746
GTGTAAT13250.023.908811
CTGTCGC18100.023.8667819
GTCTCAC28450.023.7884831
GTTCTAC8500.023.717031
TGTACCG2151.970429E-523.4412485
GTATTAG9350.023.1010021
CGCTCTG13500.022.9327355
GTTAGCC13850.022.8730493
Produced by FastQC (version 0.11.5)