FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927953
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12200.036.5898
GTCTTGC25550.033.8444481
GTCTCGC11150.032.9603961
TCGCTCT13150.029.018094
CTGTCGC17450.027.2312269
GTATAAC6300.026.307791
GTATTAG9500.025.7900621
GTGTTAG19100.025.2777581
GTGCTAT11750.025.1445031
GGTATAC6600.025.111981
GTGTTAC11450.024.5446151
GTATATA12800.024.2078151
TTGCTCT34850.023.9648064
CTCGCTC14500.023.8337273
CTTGCTC31950.023.6612193
GTTAGCC13900.022.7906443
GTATTAT8550.022.7559381
GTACTAG4750.022.7559361
TCGATCT12050.022.7046729
CGCTCTG14300.022.6566245
Produced by FastQC (version 0.11.5)