FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740713
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10700.040.372748
GTCTCGC11550.039.2716671
TCGCTCT11250.035.8390354
GTCTTGC21950.034.4409831
TCTCGCT13700.031.5319962
CTCGCTC13150.030.6607693
CGTTTCG1902.2093263E-730.314979
GTATAAC4800.029.9991911
GTCCTAC7500.029.7591971
CGCTCTG14300.027.1880765
GTATTAC7500.026.8792741
CTGTCGC16000.026.5492849
GTCTTAC9950.026.049551
ACCGTGT7850.025.6808368
GTATACG2851.0672011E-825.2624761
GTATTAT9500.025.0098511
GTATAGA9100.024.526811
GGGTTTC40750.024.3821641
GTTTACG1801.5946288E-423.9993521
TAATACT7300.023.6705934
Produced by FastQC (version 0.11.5)