FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282875
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8550.040.4203648
GTCTCGC8750.035.382251
TCGCTCT9750.031.0148564
GTCTTGC19050.030.2357061
GTCTTAC8000.027.8995271
GTGTACG2853.5106495E-1027.7889981
GTATAGC5200.027.6918351
TCTCGCT11050.027.366052
GGTATAC4500.027.1995351
GTGTTAC7800.026.7687741
CGCTCTG11400.025.8942935
TACTCCG2001.12518355E-525.1995725
CTCGCTC11300.024.8491343
GTACTAT5000.024.4795841
GTATTAG7400.024.323911
TAGTACT4450.024.269254
CTGTCGC13900.023.8269339
CTCGATC7950.023.5467688
GTATTCG2151.9700443E-523.4414621
GTCCTAC5900.023.1860471
Produced by FastQC (version 0.11.5)