FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552581
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG1105.5282726E-639.2681965
GTCTTGC20750.036.667271
TCTGTCG9800.035.9958468
GTCTCGC9000.034.6205021
TCGCTCT9950.034.0061264
GTATTAC4900.029.576131
GTATTAG7350.028.590261
GTACTAC3052.3646862E-1128.5094471
CTCGCTC11150.028.4209693
GTGTTAC9000.028.1794781
GTCTAAC4250.027.2796271
CGCTCTG11650.027.1899955
GTATTAA6650.026.1515241
GTACTAG4500.025.7640951
GTGTTAT9300.025.712151
GTGTTAG16300.025.3393021
TAACACT11400.025.2602464
GTCTTAC8050.025.2040041
TCTCGCT12150.024.8991852
TTGCTCT27050.024.485164
Produced by FastQC (version 0.11.5)