FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298989
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14850.037.8116728
GTCTCGC12350.037.626971
GTCTTGC28250.035.4688261
TCGCTCT14200.032.9515764
GGTATAC6700.030.3437611
GTGTTAC11150.029.3037931
GTATTAC7800.028.8571411
GTCTTAC11200.028.5246851
CTCGCTC15550.028.2545033
TCTCGCT15900.027.6451232
CGCTCTG16450.027.1316955
CTGTCGC23650.026.1773139
TTGCTCT38950.026.0593034
CTTGCTC34500.025.887543
GTCTCGA25850.025.3414826
GTATTAG10050.025.2864691
GTATTAA9300.024.9834981
GTGTTAG20900.024.6659741
TGTTAGC18400.024.2806152
GGGTTTC43100.024.2589171
Produced by FastQC (version 0.11.5)