FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565517
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC27700.036.2428361
TCTGTCG12150.036.141248
GTCTCGC13450.031.9118961
GTCTTAC12750.029.6698951
GTATTAC8200.028.389581
GTATTAG12500.026.7713761
TTACTCT17100.026.5212444
GTACCGT1658.841985E-526.1768136
TCGCTCT14650.026.0428034
GTATTAA8700.025.085621
GTGTTAG22550.024.8408831
GTGTAAC10700.024.4760671
GTACTAA6700.023.8875031
TTGCTCT38050.023.6485654
CTCGCTC15550.023.1466983
TCTGTCA40250.022.8925178
CTGTCGC22100.022.8010719
TTATACT9550.022.6134784
CTTGCTC36700.022.5569933
CTTACTC15850.022.254423
Produced by FastQC (version 0.11.5)