FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565517
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14000.034.9672478
GTCTTGC28600.031.7479321
GTCTCGC13900.030.8324951
GTGTTAC11500.028.4240631
GTCTTAC12400.026.9468291
GTATAGT9300.026.5562931
GCGTTAC1658.315245E-526.414281
TCGCTCT15950.026.1786864
TTGCTCT40650.025.8566864
GTGCTAC11400.024.8502771
TAATACT10200.024.7029274
TATACTG11750.024.5077085
GTGTTAG20600.024.330631
CTTGCTC40550.023.9739283
GTATATA13950.023.9527341
CTCGCTC17400.023.5896723
ACCGTGT11300.023.5724018
GTTTCAC42800.023.5548633
GTACTAT6550.023.2889271
CGCTCTG18250.023.273935
Produced by FastQC (version 0.11.5)