FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3876569
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13200.034.9024168
GTCTCGC14450.030.692291
GTCTTGC30500.030.5124721
GTATTAT10750.027.053211
GTATAAT11600.026.3244041
GTGTTAC12900.025.9259911
TCGCTCT14500.025.8157544
GTATTAA10750.025.024221
GTATTAC9100.024.7678071
TCTCGCT16850.024.3514272
GTCTCAC38600.023.921241
TAGTACT7600.023.6796864
GTCGTAC1600.002140760222.7204691
CTCGCTC16800.022.7099463
CTGTCGC21650.022.6102879
GTGTAAC11300.022.5194021
TTACTCT20800.021.8035244
GTCTAAG12700.021.75441
GTGTTAG24100.021.7211461
GTGTTAT14750.021.6884211
Produced by FastQC (version 0.11.5)