FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662274
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC29350.033.2034451
TCGCTCT13900.030.5538394
TCTGTCG13300.030.3085358
GTCTCGC13600.029.945781
GGTATAC7550.028.8975351
TCTCGAT13200.025.6302287
TCGATCT13300.024.8962979
CTCGCTC17150.024.7637543
GTATTAA10000.024.7266581
CTCGATC13450.024.6186438
TAATACT9150.024.3876064
TCTCGCT17350.024.0634042
GTGTAAA20250.023.7031141
GTATAAT10550.023.437591
GTATAAA13400.023.3372761
TTATACT9000.023.1944484
GTACTAT7600.022.9659371
GTATTAT10800.022.8950541
CGCTCTG16900.022.5744785
TACACTG19800.022.176515
Produced by FastQC (version 0.11.5)