FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3824059
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13600.031.7576358
GTCTTGC30350.031.3811191
GTCTCGC13500.026.3887061
GTGTAAC10050.026.0430741
GTATTAG12600.025.9655651
TCGCTCT15500.025.54274
GTATTAC8300.023.6505621
GTACTAC6550.023.3095461
GTATAAC6700.022.787691
GTCTTAC12150.022.738571
TTGCTCT42550.022.1619284
GTGTAGC16750.022.1366141
GTATAAT12500.022.1018891
CTTGCTC42150.022.0306823
TCTCGCT17150.021.8260462
CGCTCTG17550.021.3285855
GTGTTAC14000.021.2917631
CTGTCGC21650.021.2793259
GTCTTAG15050.021.2555331
GTCTCAC37700.021.2132471
Produced by FastQC (version 0.11.5)