FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3824059
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15100.037.5182231
TCTGTCG14700.035.749398
TCGCTCT15050.032.0480354
GTCTTGC29050.029.5026131
GTATAAC6900.028.4209961
CTCGCTC18800.025.6638663
GTCGTAC1701.0194115E-425.6346241
CGCTCTG17900.024.9345665
TCTCGCT21200.024.8043862
GTATTCG2351.3112276E-624.7255951
GTACTAT8000.024.5131071
GTACTAA7850.023.1310311
ATACCGT2252.8032644E-522.396426
GTCTCAC33450.021.9305671
GTATTAG12300.021.848481
GGAGTGC66000.021.596555
GTATTAT11800.021.5432221
GTATTAA10500.021.4435651
GTGTTAC13300.021.2979371
GAGTGCA73000.021.1034776
Produced by FastQC (version 0.11.5)