FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005350031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005350031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520731
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14700.035.7473758
GTCTTGC33800.033.9761961
GTCTCGC15250.033.8394171
TCGCTCT17100.029.4673124
TCTCGCT18000.027.5940322
CTCGCTC18750.025.7224393
CTATCGA2001.1271484E-525.1945519
GGGTTTC53600.024.2729511
GTGTTAG23700.024.227751
CGCTCTG19950.024.1752245
GTGTAAC12050.023.524041
GTCTTAC14600.023.3980331
GTATTAA8700.023.3923111
CTGTCGC22800.023.047659
GTTTCAC48000.022.945043
TTGCTCT46100.022.6415254
TAGTACT7650.022.5833534
CTTGCTC45750.022.3427093
GTATTAT8800.022.3005431
CGTTTCG1952.7459796E-422.1490559
Produced by FastQC (version 0.11.5)