FastQCFastQC Report
Thu 16 May 2024
EGAF00005394161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527351022
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCAGC22876070943.37921032795495No Hit
CGATAGCT10701890920.29367622994765No Hit
GTCAGCTA9690034618.37492333522016No Hit
AATGTTAG8879025416.83703080033094No Hit
TCGCCAGA9502760.18019800101951827No Hit
AATGGTAG7700550.14602323080356142No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)