FastQCFastQC Report
Thu 16 May 2024
EGAF00005394164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519960750
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTCACG15206899229.24624445210528No Hit
CACGAGAA14288664427.480275001526554No Hit
GTAATTGC9320031717.92449083897198No Hit
AGTCGCTT6859159613.191687257163162No Hit
AGTCGCGT5861676811.273306302446867No Hit
TCGTCACA6633730.12758136070847656No Hit
AGTCGCTA6460070.12424149322809463No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)