FastQCFastQC Report
Thu 16 May 2024
EGAF00005394176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579106580
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGGA17386489530.022952769764764No Hit
GCTGAATT15939260827.52388135531114No Hit
ATGCTCCG13027705322.496213564004055No Hit
TGAAGTAC11093295519.15587887120882No Hit
CACTCGAA7642360.13196810853021218RNA PCR Primer, Index 47 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)