FastQCFastQC Report
Wed 8 May 2024
EGAF00005394215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59591346
Total Bases476.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCACT1985993833.32688273226787No Hit
GTCGATGC1438715124.143020699683472No Hit
TGATTCTA1183265219.856326118225287No Hit
ACTAGGAG1146105819.232755709193075No Hit
ACTCGGAG12748122.139256931702801No Hit
TGAATCTA2060570.3457834296946406No Hit
CAGCCACA1288820.2162763700621899No Hit
ACGAGGAG935260.15694560750482126No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)