FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005394228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39433218
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT557336714.13368546285013No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA6765091.7155815180997909No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT4533081.1495587299012726No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT3100480.7862609640430562No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA2960470.7507553656919402No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC2651810.6724812567921796No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT2109010.5348308119311997No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG1832420.4646894402582107No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG1762860.4470494901024817No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC1658690.42063267573039564No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT1295110.3284312226306258No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG1214570.30800681800810675No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1062990.26956714514143887Clontech SMART CDS Primer II A (100% over 26bp)
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG957890.24291448899757562No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT787860.19979601969080993No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC706620.17919409975620046No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG705960.17902672817622947No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT652110.16537072881041565No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC607830.15414161735418092No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC564510.1431559554688131No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG541940.13743235462041165No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC507960.12881525418493617No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG494220.125330882201904No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGC468390.1187805671857671No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA458250.1162091310934857No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT454920.11536466539454122No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA446010.11310514906493303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)