FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005394234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46179747
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44782049.697333335325549No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC9286632.0109746378645164No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT5584341.2092617137984754No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT3990450.8641125729857291No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT3122970.6762639907923272No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA3014490.6527731734866369No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA2984450.6462681573374579No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGC2935440.63565527979181No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC2914760.6311771261977681No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCG2730320.5912375396946198No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG2187410.4736730151423307No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT1456470.3153915070171346No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG1310000.28367413966126753No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG1156240.25037815819995723No Hit
GCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA1095830.23729666600382196No Hit
CTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCAC1060400.22962447152428098No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG979200.212041005768178No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT892450.19325571445854825Clontech SMART CDS Primer II A (100% over 26bp)
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC883680.1913566135388312No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG871140.1886411374232951No Hit
GTCATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG796690.17251935139445437No Hit
GCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTTAG762630.16514382376326142No Hit
GTTGGTACCAGAAGACATAGTCATAACCACCAACGTCACTGCTGGTTCCA760960.1647821933714795No Hit
GTCAGCTTGGTCCCTCCGCCGAATATCACGAAATTGTTGCTGCCTGCATA672060.14553133000057364No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC660020.14292412645742733No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC643730.13939660604896775No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC632110.13688035146662886No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAG629020.1362112269692599No Hit
GTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGAAGACATAGTCATAA599580.12983613790694867No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA596550.12918000611826652No Hit
GCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTTAGTGACCTCATAAA591420.1280691295255472No Hit
GAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCC584340.12653598990050768No Hit
GGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGCCCTGAGTGAGGAG583390.12633027201296707No Hit
GTAATAATTAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG578740.12532333708974197No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC575620.12464771623802963No Hit
GTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTA575300.12457842179170014No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA569150.12324666915130567No Hit
AGAAGACATAGTCATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAG546120.11825963446703161No Hit
GCCGCTTAGTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGG544170.11783737143471142No Hit
CTTTGCCTGGGTGGTGTTGGTACCAGAAGACATAGTCATAACCACCAACG535430.11594476686933777No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC531870.1151738661539224No Hit
GATCAGGGACCCCTGAGGGCCGCTTAGTGACCTCATAAATCATGAGTTTG529930.11475376857305No Hit
ATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGAAGACATAGTC526440.11399802601776922No Hit
GTCAGGGCAGACTGGGCCCAGGACCCTGTGCCCTGAGTGAGGAGGGTGAG519440.11248221000431206No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG504820.10931631998763441No Hit
GGGACCCCTGAGGGCCGCTTAGTGACCTCATAAATCATGAGTTTGGGGGC491730.10648174404246952No Hit
TGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC484200.1048511591022792No Hit
GCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACG478180.10354755733070603No Hit
GGTGTTGGTACCAGAAGACATAGTCATAACCACCAACGTCACTGCTGGTT472420.10230025729677558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)