FastQCFastQC Report
Wed 8 May 2024
EGAF00005394235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77844970
Total Bases622.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGACAT2570059833.01510425143718No Hit
CGATCACC2172846327.912481692779895No Hit
GATCTTGA1601993120.579275706574233No Hit
TTCAGGTG1339039417.20136060171903No Hit
GATCTAGA2450720.3148205979140335ABI Dynabead EcoP Oligo (100% over 8bp)
GATCTCGA1652580.21229117308414402No Hit
CGATCACA1139830.14642307653275477No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)