FastQCFastQC Report
Sat 25 May 2024
EGAF00005394237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77844970
Total Bases11.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT816479210.48852867436393No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC13944251.7912846520462402No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT8148681.0467831126404183No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT6049900.7771728860580202No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA5543630.7121372132329167No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC4274040.5490451085021936No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGC4264310.547795188308249No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT4134700.5311454291780188No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCG3994470.5131314200519315No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA3273560.4205229958981293No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG2931020.3765201528114148No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT2308530.296554806302835No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC1740130.22353788562061236No Hit
GCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA1699780.21835450639906473No Hit
CTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCAC1628830.2092402373589456No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC1581030.20309982777307256No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG1580610.20304587438340588No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG1483190.1905312571897709No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC1433340.1841275036781439No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1424360.1829739288228899No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1398030.17959156513259622Clontech SMART CDS Primer II A (100% over 26bp)
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG1347000.1730362282880962No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG1344650.17273434622686604No Hit
GTCATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG1314820.16890237095601682No Hit
GTTGGTACCAGAAGACATAGTCATAACCACCAACGTCACTGCTGGTTCCA1202230.15443900871180244No Hit
GCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTTAG1187350.15252751719218338No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC1183170.15199055250454846No Hit
GGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGCCCTGAGTGAGGAG1105050.14195522202654842No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA1097670.14100718389383413No Hit
GTCAGCTTGGTCCCTCCGCCGAATATCACGAAATTGTTGCTGCCTGCATA1052500.13520462529563568No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT1029170.13220764296010393No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC1018640.13085495440488962No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC1003810.12894988590784992No Hit
GTCAGGGCAGACTGGGCCCAGGACCCTGTGCCCTGAGTGAGGAGGGTGAG983150.12629589297805627No Hit
GTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGAAGACATAGTCATAA942890.12112407519715147No Hit
GCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGCTTAGTGACCTCATAAA916890.11778410345588161No Hit
GTAATAATTAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG915420.11759526659204826No Hit
GTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTA905820.11636204625681017No Hit
GAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCC899980.11561183721954033No Hit
AGAAGACATAGTCATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAG869420.11168608581903236No Hit
GCCGCTTAGTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGG856900.11007776096515934No Hit
ATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTACCAGAAGACATAGTC841290.10807249331588155No Hit
CTTTGCCTGGGTGGTGTTGGTACCAGAAGACATAGTCATAACCACCAACG836850.10750212891083392No Hit
GATCAGGGACCCCTGAGGGCCGCTTAGTGACCTCATAAATCATGAGTTTG828970.10648986055232598No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAG810360.1040992115482863No Hit
GCTGGTTCCAGTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACG799000.10263990081825454No Hit
GGGACCCCTGAGGGCCGCTTAGTGACCTCATAAATCATGAGTTTGGGGGC778770.10004114588264341No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)