FastQCFastQC Report
Thu 12 May 2022
EGAF00005394240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44775447
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT700133115.6365407139319No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA4564161.0193443741611334No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC2918280.6517589874647148No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC2884600.6442370078404801No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG2630580.5875050225629238No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT2458190.5490040110599007No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1715530.3831407869585311No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG1701720.38005650730857027No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC1331160.2972968645070143No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT1273790.2844840387634768No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1177390.2629543821192896No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1146890.25614261316028847Clontech SMART CDS Primer II A (100% over 26bp)
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC984730.21992633596712055No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC922230.20596779301834778No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA906990.20256414190571898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG879820.19649608411502847No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC751170.1677638193092746No Hit
GGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAGCTG717890.16033117435991204No Hit
GTTGGTACCAGGCTAAGTAGCTGCTAACACTCTGACTGGCCCTGCAGGAG675390.15083936515474652No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG628050.14026660638362806No Hit
GGTTTCTGTTGGTACCAGGCTAAGTAGCTGCTAACACTCTGACTGGCCCT604650.13504052790360754No Hit
GAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCC592350.13229348665128904No Hit
GCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGGAGACTGTGTCAACAC576580.1287714670944547No Hit
CCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGG570960.12751631491250104No Hit
GCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTG553890.1237039576623322No Hit
GCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCC546220.12199096527165881No Hit
GTAGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC532710.1189736866278521No Hit
CTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGA512990.1145694871566553No Hit
GTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGT506130.113037397482598No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG490420.10952877812699446No Hit
GTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGGAGACTGTGTCAA473730.10580128881795417No Hit
GTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCC469670.1048945418680019No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)