FastQCFastQC Report
Thu 12 May 2022
EGAF00005394246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39150616
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT475461012.144406616743911No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA12399173.1670433997769023No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG4860621.2415181411194143No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG3906970.9979332125961952No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG3648900.9320159866705545No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT3199300.8171774359821057No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA2971510.7589944434079913No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC2923470.7467238829652131No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2264140.5783152939407135Clontech SMART CDS Primer II A (100% over 26bp)
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1988730.5079690189293573No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1782030.45517291477610466No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG1727050.4411297130037494No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT1307870.33406115500200556No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG1220670.3117881976620751No Hit
GGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTGGG1187980.3034383928978282No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC1069760.27324218857756927No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC912060.23296185173689224No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC818240.20899798869065048No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG751950.19206594348349462No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC654170.1670906020993386No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA602160.15380600908041908No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT558350.14261589140768563No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC537560.1373056301336357No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG440170.11242990404033489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)