FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58709938
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC255650.018.7959291
ACTGAAC168600.018.1781861
ACTGATG159600.017.8522871
CTCGAAA161300.017.848281
GACCTGG211300.017.7638261
CAATGGA191800.017.7613498
CGAGCAC185400.017.6998861
TGACAAC187950.017.6997241
ACGAGCC171500.017.6911931
GATGAAA178950.017.5940151
GGGACCT146650.017.5533811
TGCGGGT253400.017.4694141
GGGAATG145800.017.3840891
GCGCGAT131000.017.3494631
CTGCCTA199650.017.2630711
ATCGAGT166650.017.2290841
GGCGACT167400.017.224181
GACTAAC135800.017.206071
GCCGACA144850.017.2005923
TGCACCT251150.017.183521
Produced by FastQC (version 0.11.5)